Novel Coronavirus COVID-19 (2019-nCoV) Real Time Multiplex RT-PCR Kit (Detection for 3 Genes )

Supplier Liferiver · Catalog number: RR-0479-02

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Price

469.56 EUR

Size
25 tests/kit
Novel Coronavirus (2019-nCoV) Real Time Multiplex RT-PCR Kit is used for the qualitative detection of a novel coronavirus, which was identified in 2019 at Wuhan City, Hubei Province, China, in upper respiratory tract specimens (nasopharyngeal extracts, deep cough sputum, etc.) and lower respiratory tract specimens (alveoli irrigation fluid, etc.) by real time PCR systems. It detects N gene, E gene and RdRp gene of 2019-nCoV. RR-0479-02 has been also approverd by CFDA for emergency use and is WHO standard.

User Manual

RR-0479-02 Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit

Material Safety Data Sheet

Material Safety Data Sheet RR-0478-02 and RR-0479-02 Novel Coronavirus (2019-nCoV) Real Time RT-PCR Kit
  • Specifications:Analysis sensitivity: 1×10^3 copies/ml

    On January 11, 2020, Chinese health authorities preliminarily identified more than 40 human infections with a novel coronavirus in an outbreak of pneumonia under investigation in Wuhan City, Hubei Province, China. The Chinese authorities identified a new type of coronavirus (novel coronavirus, named as 2019-nCoV), which was isolated on 7 January 2020. Coronaviruses are a large family of viruses, some causing illness in human and others circulating among animals such as camels, cats and bats. 2019-nCoV is a novel coronavirus. The primer and probe design for this kit is based on the newly released strain (2019-nCoV) (GeneBank accession: MN908947) and covers 6 2019-nCoV strains sequences (EPI_ISL_402119, EPI_ISL_402120, EPI_ISL_402121, EPI_ISL_402122, EPI_ISL_402123, EPI_ISL_402124 included). The kit contains a specific ready-to-use system for the detection of Novel Coronavirus (2019-nCoV) by Reverse transcription Polymerase Chain Reaction (RT-PCR) in the real-time PCR system. The master contains a Super Mix for the specific amplification of virus RNA. The reaction is done in one step real time RT-PCR. The first step is a reverse transcription (RT), during which the virus RNA is transcribed into cDNA. Afterwards, a thermostable DNA polymerase is used to amplify the specific gene fragments by means of polymerase chain reaction (PCR). Fluorescence is emitted and measured by the real time systems' optical unit during PCR. The detection of amplified virus DNA fragment is performed in fluorimeter channel FAM, HEX/VIC/JOE and Cal Red 610/ROX/TEXAS RED with the fluorescent quencher BHQ1.

  • Storage and shipping:
    • Shipping: dry ice
    • All reagents should be stored at -20°C. Storage at +4°C is not recommended.
  • Additional informations: For use with PE5700, MJ-Opticon & other single color systems, ABI7000, ABI7300, ABI7500, ABI7900, ABI StepOne, StepOne plus, MJ-Opticon2, MJ-chromo4, MX3000P, MX3005P, Smart Cycler II, Rotor-Gene 6000, LightCycler 480, CFX 96, Life 96, Slan 96, iCycler iQ4, iCycler iQ5 & other multi-color systems

* The price of the product is approximate, please contact us for price confirmation

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