A sandwich ELISA kit for detection of Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha from Rat in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPAR?C1?). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPAR?C1?). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPAR?C1?), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
- 10nm. The concentration of Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPAR?C1?) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The Rat PPARgC1a ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
- Species reactivity: Rat
- Sensitivity: 0.127ng/mL
- Detection range: 0.312-20ng/mL
- Standard solution concentration: 20ng/mL
- ELISA type: Sandwich
- Assay length: 3h
This kit is intended for research use only. Do not use for diagnostics purposes.
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