A competitive Inhibition ELISA kit for detection of Fibrinopeptide B from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
A monoclonal antibody specific to Fibrinopeptide B (FPB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibrinopeptide B (FPB) and unlabeled Fibrinopeptide B (FPB) (Standards or samples) with the pre-coated antibody specific to Fibrinopeptide B (FPB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibrinopeptide B (FPB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibrinopeptide B (FPB) in the sample.
The Human FPB ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
- Species reactivity: Human
- Sensitivity: 162.7pg/mL
- Detection range: 370.37-30000pg/mL
- Standard solution concentration: 30000pg/mL
- ELISA type: Competitive Inhibition
- Assay length: 2h
This kit is intended for research use only. Do not use for diagnostics purposes.
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