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 "ELISA kit for Rabbit NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide)")
ELISA kit for Rabbit NT-ProBNP (N-Terminal Pro-Brain Natriuretic Peptide)
Supplier ELK Biotech · Catalog number: ELK2957
Price
631.20 EUR
Size
1 plate of 96 wells
Description
- Species reactivity: Rabbit
- Sensitivity: 12.4pg/mL
- Detection range: 31.2-2000pg/mL
- Standard solution concentration: 2000pg/mL
- ELISA type: Sandwich
- Assay length: 3h
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
- 10nm. The concentration of N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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