A competitive Inhibition ELISA kit for detection of Glucose 6 Phosphate Isomerase from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
A monoclonal antibody specific to Glycophosphatidylinositol (GPI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Glycophosphatidylinositol (GPI) and unlabeled Glycophosphatidylinositol (GPI) (Standards or samples) with the pre-coated antibody specific to Glycophosphatidylinositol (GPI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glycophosphatidylinositol (GPI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Glycophosphatidylinositol (GPI) in the sample.
The Human GPI ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.
- Species reactivity: Human
- Sensitivity: 82.3pg/mL
- Detection range: 246.91-20000pg/mL
- Standard solution concentration: 20000pg/mL
- ELISA type: Competitive Inhibition
- Assay length: 2h
This kit is intended for research use only. Do not use for diagnostics purposes.
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