Image of ELISA kit for Rat PKM2 (Pyruvate Kinase, Muscle)

ELISA kit for Rat PKM2 (Pyruvate Kinase, Muscle)

Supplier ELK Biotech · Catalog number: ELK1515

Price

432.00 EUR

Size
1 plate of 96 wells
Description
  • Species reactivity: Rat
  • Sensitivity: 0.42ng/mL
  • Detection range: 0.94-60ng/mL
  • Standard solution concentration: 60ng/mL
  • ELISA type: Sandwich
  • Assay length: 3h
This kit is intended for research use only. Do not use for diagnostics purposes.
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Kinase, Muscle (PKM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Kinase, Muscle (PKM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Kinase, Muscle (PKM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
  • 10nm. The concentration of Pyruvate Kinase, Muscle (PKM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The Rat PKM2 ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.

* The price of the product is approximate, please contact us for price confirmation

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