Image of ELISA kit for Human C5a (Complement Component 5a)

ELISA kit for Human C5a (Complement Component 5a)

Supplier ELK Biotech · Catalog number: ELK1051

Price

372.00 EUR

Size
1 plate of 96 wells
Description
  • Species reactivity: Human
  • Sensitivity: 27pg/mL
  • Detection range: 78-5000pg/mL
  • Standard solution concentration: 5000pg/mL
  • ELISA type: Sandwich
  • Assay length: 3h
This kit is intended for research use only. Do not use for diagnostics purposes.
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 5a (C5a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 5a (C5a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 5a (C5a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±
  • 10nm. The concentration of Complement Component 5a (C5a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
The Human C5a ELISA kit is shipped on ice packs. Upon receipt keep refrigerated at + 4 °C.

* The price of the product is approximate, please contact us for price confirmation

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