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Mouse E-Cad(E-Cadherin) ELISA Kit

Supplier Elabscience Biotechnology · Catalog number: E-EL-M0211-96

Price

367.00EUR

Size
96 tests

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse E-Cad. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse E-Cad and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse E-Cad, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse E-Cad. You can calculate the concentration of Mouse E-Cad in the samples by comparing the OD of the samples to the standard curve.

  • Additional information:No significant cross-reactivity with analogues from other species was determined. Please, note that the data on cross-reactivity is limited. Other samples, aside from the tested ones, may be suitable to be used with this kit.
  • Storage and shipping:Shipped on ice packs. Upon receipt store refrigerated at +4 deg. C.
  • Notes:For research use only.

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