ELISA kit for Human APAF1 (Apoptosis Protease Activating Factor 1)

Description

• Species reactivity: Human
• Antigen: APAF1
• Assay type: Sandwich ELISA
• Sensitivity: 0.1ng/mL
• Detections range: from: 0.16 to: 10ng/mL
• Validated sample types: Serum, Plasma, Cell supernatant

This Human APAF1 ELISA kit is intended for research use only. Do not use for human or animal diagnostics. Gentaur's APAF1 ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human APAF1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human APAF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human APAF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human APAF1. You can calculate the concentration of Human APAF1 in the samples by comparing the OD of the samples to the standard curve. The kit is shipped on ice packs or blue ice. Upon receving it, keep refrigerated at 1 to 8 degress Celsius. The shelf life of this is guaranteed to be at least 6 months from the date of receipt.