Image of ELISA kit for Human TNF-? (Tumor Necrosis Factor Alpha)

ELISA kit for Human TNF-? (Tumor Necrosis Factor Alpha)

Supplier Elabscience Biotech · Catalog number: E-EL-H0109


377.00 EUR

1 plate of 96 wells
  • Species reactivity: Human
  • Antigen: TNF-?
  • Assay type: Sandwich ELISA
  • Sensitivity: 4.69pg/mL
  • Detections range: from: 7.81 to: 500pg/mL
  • Validated sample types: Serum, Plasma, Cell supernatant
This Human TNF-? ELISA kit is intended for research use only. Do not use for human or animal diagnostics. Gentaur's TNF-? ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-?. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-? and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-?, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TNF-?. You can calculate the concentration of Human TNF-? in the samples by comparing the OD of the samples to the standard curve. The kit is shipped on ice packs or blue ice. Upon receving it, keep refrigerated at 1 to 8 degress Celsius. The shelf life of this is guaranteed to be at least 6 months from the date of receipt.

* The price of the product is approximate, please contact us for price confirmation

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