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6-keto-PGF1a(6-keto-prostaglandin F1a) ELISA Kit

Supplier Elabscience Biotechnology · Catalog number: E-EL-0054-96



96 tests

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 6-keto-PGF1a. During the reaction, 6-keto-PGF1a in the sample or standard competes with a fixed amount of 6-keto-PGF1a on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 6-keto-PGF1a. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 6-keto-PGF1a in the samples is then determined by comparing the OD of the samples to the standard curve.

  • Additional information:No significant cross-reactivity with analogues from other species was determined. Please, note that the data on cross-reactivity is limited. Other samples, aside from the tested ones, may be suitable to be used with this kit.
  • Storage and shipping:Shipped on ice packs. Upon receipt store refrigerated at +4 deg. C.
  • Notes:For research use only.

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