Image of ELISA kit for 25-HVD3 (25-Hydroxy Vitamin D3)

ELISA kit for 25-HVD3 (25-Hydroxy Vitamin D3)

Supplier Elabscience Biotech · Catalog number: E-EL-0015


377.00 EUR

1 plate of 96 wells
  • Species reactivity: Universal
  • Antigen: 25-HVD3
  • Assay type: Competitive ELISA
  • Sensitivity: 1.88ng/mL
  • Detections range: from: 3.13 to: 200ng/mL
  • Validated sample types: Serum, Plasma, Cell supernatant
This Universal 25-HVD3 ELISA kit is intended for research use only. Do not use for human or animal diagnostics. Gentaur's 25-HVD3 ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 25-HVD3. During the reaction, 25-HVD3 in the sample or standard competes with a fixed amount of 25-HVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 25-HVD3. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 25-HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve. The kit is shipped on ice packs or blue ice. Upon receving it, keep refrigerated at 1 to 8 degress Celsius. The shelf life of this is guaranteed to be at least 6 months from the date of receipt.

* The price of the product is approximate, please contact us for price confirmation

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