Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml;_x000D_Immunohistochemistry (Frozen Section), 0.5-1μg/ml;_x000D_Immunocytochemistry, 0.5-1μg/ml;_x000D_Flow Cytometry, 1-3μg/1x106 cells;_x000D_Direct ELISA, 0.1-0.5μg/ml
Human
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
- Clonality: Polyclonal
- Ig type: Rabbit IgG
- Form: Lyophilized
- Specificity: No cross reactivity with other proteins.
- Reactivity: Reacts with: human
- Application: IHC-P,IHC-F,ICC,FCM,Direct ELISA
- Notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- Immunogen: E. coli-derived human ICOS recombinant protein (Position: E21-K140).
- Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
- Purification: Immunogen affinity purified.
- ReconstitutionAdd 0.2ml of distilled water will yield a concentration of 500μg/ml.
- Gene Name: ICOS
- Protein Name: Inducible T-cell costimulator
- Gene Full Name: inducible T cell co-stimulator
- Uniprot ID: Q9Y6W8
- Entrez GeneID: 29851
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