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Herpes Simplex 2 IgM ELISA kit

Supplier Fitzgerald · Catalog number: 55R-IB79249


316.00 EUR

96 wells
  • Category: Research Kits
  • Research Area: Infectious Disease
  • Specificity: NA
  • Form & Buffer: NA
  • Applications: ELISA
  • Usage Recommendations: Optimal conditions to be determined by end user
  • Assay Information: This Herpes 2 IgM antibody test kit is based on the principle of the enzyme immunoassay (EIA). Herpes 2 antigen is bound on the surface of the microtiter strips. Diluted patient serum or readytouse standards are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Herpes 2 antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then readytouse antihumanIgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgM antibodies is directly proportional to the intensity of the color. NOTE that samples containing hyperimmune levels of antihuman IgG antibodies may cause false positive results in this assay.  Any patient samples testing positive should be prediluted with IgG adsorbent and retested to confirm elevated levels of IgM.
  • Storage: Store at 2-8°C
  • Shipping: Blue Ice

* The price of the product is approximate, please contact us for price confirmation

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