It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
- Tested Applications: E, WB
- Applications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 28.2 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- Predicted Molecular Weight: 28.2 kDa
- Physical state: Lyophilized
- Buffer: PBS, pH7.4
- Concentration: N/A
- NCBI official symbol: N/A
- Accession #: N/A
- Protein GI: N/A
- NCBI gene ID#: N/A
- NCBI official full name: N/A
- NCBI organism: N/A
- Peptide sequence: N/A
- SWISSPROT #: N/A
- Background Reference 1: Wan Y, et al. J Virol. 2020. pii: JVI.00127-20.
- Background Reference 2: Benvenuto D, et al. J Med Virol. 2020.
- Background Reference 3: Chang CY, et al. AMB Express. 2020. 10(1):20.
- Background Reference 4: N/A
- Background Reference 5: N/A
- Source: HEK293 cells
- Species: MERS
- By Source: Human Cells
- By Species: Other
- Fusion tag: His Tag
- Sequence: Glu 367 - Tyr 606
- Biology activity: N/A
- Purity: >90% as determined by SDS-PAGE.
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