They are designed for the in vitro sensitive quantitative determination of human insulin concentrations in serum, plasma, cell culture super mates, and other biological fluids.
Primarily, insulin is responsible for controlling glucose metabolism. Insulin is metabolized in the β-cells of the islets of Langerhans in the pancreas. Reduced levels of insulin lead to insulin-dependent diabetes mellitus (IDDM). CLIA detection using a micro-plate provides a more sensitive, high throughput and an economical alternative to other alternatives such as conventional colorimetric methodologies.
In addition, CLIA does not require long incubations or the addition of stopping reagents. The kits are able to detect glow-based chemiluminescence reactions and they provide a broader dynamic assay range, have superior low-end sensitivity, and a faster protocol. Moreover, CLIA can be used for diagnostic and research areas where other convention colorimetric cannot be used.
The human insulin CLIA kits are valid and are based on a solid phase enzyme-linked immunosorbent assay that utilizes one anti-insulin antibody for solid-phase immobilization and an additional anti-insulin antibody in the antibody-enzyme conjugate solution. The increasing need to detect tiny amounts of target analyte molecules in liquids led to the development of chemiluminescence immunoassay. It increased the analytical sensitivity of immunoassays.
CLIA kits are not enzyme-linked, they utilize non-enzymatic reporter biochemistry techniques. Consequently, CLIA avoids the use of radioactive techniques, however, they may incorporate both enzymatic and chemiluminescent technology.
Chemiluminescent detection technology in CLIA assays provides perfect solutions for researchers who need immunoassays with high sensitivity, wide linear range, and limited sample volume. The wide kinetic linear range allows for the detection of analytes with both low and high concentrations in a cohort sample.
CLIA enables the researcher to detect the target analyte molecule in different samples that have widely differing concentrations using the same dilution fold for all the samples, as a consequence, it reduces assay variability and is more convenient.