GENTAUR antibody-antibodies.com The Marketplace for Antibodies : Nimbolide induced apoptosis by activating ERK-mediated inhibition of c-IAP1 expression in human hepatocellular carcinoma cells.

Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :29962003
Publication Date : //

Nimbolide induced apoptosis by activating ERK-mediated inhibition of c-IAP1 expression in human hepatocellular carcinoma cells.

Nimbolide is one of the major compounds from the leaves and flowers of the neem tree and exhibits antitumor properties on various cancer cells. However, no report has shown that nimbolide induces apoptosis in vitro and in vivo in human hepatocellular carcinoma cells. Our results indicated that it inhibited cell growth in Huh-7 and PLC/PRF/5 cells. We also found that nimbolide induced cell death through the induction of G2/M phase arrest and mitochondrial dysfunction, accompanied by the increased expression of cleaved caspase-7, caspase-9, caspase-3, caspase-PARP, and Bax and decreased expression of Mcl-1 and Bcl-2. A human apoptosis antibody array analysis demonstrated that inhibition of the apoptosis family proteins (XIAP, c-IAP1, and c-IAP2) was one of the major targets of nimbolide. Additionally, nimbolide sustained activation of ERK expression. Moreover, pretreatment with U0126 (MEK inhibitor) markedly abolished nimbolide-inhibited cell viability, induced cell apoptosis, ERK phosphorylation, cleaved caspase-9, caspase-3, cleaved-PARP activation, and increased c-IAP1 expression in Huh-7 cells. An in vivo study showed that nimbolide significantly reduced Huh-7 tumor growth and weight in a xenograft mouse model. This study indicated the antitumor potential of nimbolide in human hepatocellular carcinoma cells.

Authors : Hsueh Kuan-Chun , Lin Chia-Liang , Tung Jai-Nien , Yang Shun-Fa , Hsieh Yi-Hsien ,

Related products :

Catalog number	Product name	Quantity

EIAAB46725	HCCA2,Hepatocellular carcinoma susceptibility protein,Hepatocellular carcinoma-associated protein 2,Homo sapiens,Human,YY1AP,YY1AP1,YY1-associated protein 1
30-121	C21orf33 inhibits both estrogen- and heregulin-beta1-stimulated growth of breast cancer cells, and further suggests that growth inhibition induced in these cells by all-trans retinoic acid occurs via	0.1 mg
31-015	DIABLO is an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. M	0.1 mg
HEPG2-500	HepG2 Human Hepatocellular Carcinoma Cells	500,000 cells
27-475	SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediate	0.05 mg
27-476	SMAD5 undergoes copy number gain and increased expression, rather than loss of expression, and therefore does not act as a tumor-suppressor gene in hepatocellular carcinoma. Up-regulated Smad5 mediate	0.1 mg
EIAAB32890	HCCA3,Hepatocellular carcinoma-susceptibility protein 3,Homo sapiens,Human,PAC2,PAC-2,Proteasome assembly chaperone 2,PSMG2,TNFSF5IP1,Tumor necrosis factor superfamily member 5-induced protein 1
27-186	TXNDC5 is a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis.This gene encodes a protein-disulfide isomerase. Its expressio	0.05 mg
EIAAB43671	C20orf1,C20orf2,Differentially expressed in cancerous and non-cancerous lung cells 2,DIL2,DIL-2,HCA519,Hepatocellular carcinoma-associated antigen 519,Homo sapiens,Human,p100,Protein fls353,Restricted
31-023	TNFSF6 is a cytokine that binds to TNFRSF6_FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T cell mediated apoptosis and in T cell development. TNFRSF6_FA	0.1 mg
26-210	LAX1 is a single-pass type III membrane protein. It negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and BCR (B-cell antigen receptor)-mediated signaling in B-cells.	0.05 mg
29-961	Transport of folate compounds into mammalian cells can occur via receptor-mediated or carrier-mediated mechanisms. A functional coordination between these 2 mechanisms has been proposed to be the meth	0.1 mg
31-081	PDCD4 is a protein localized to the nucleus in proliferating cells. Expression of its gene is modulated by cytokines in natural killer and T cells. PDCD4 is thought to play a role in apoptosis but the	0.05 mg
HCL-1211	Human HepG2 (Hepatocellular Carcinoma) lysate	100ug
E4231h	Human Hepatocellular Carcinoma Related Protein 1 E	96T
CS03-01-003	Human Hepatocellular carcinoma tissue array 5 (grade I ~ III)	7 x 9
HTS-10222	Human Liver Slide (Hepatocellular Carcinoma) (5 slides_pk)	1 pk
CS03-01-003	Human Hepatocellular carcinoma tissue array 5 (grade I ~ III)	7 x 9
TS1075	Human hepatocellular carcinoma (100 cases) Tissue Section	1 slide
Y050959	Anti-Human Hepatocellular Carcinoma [CHALV2] antibody	250ug
Y050958	Anti-Human Hepatocellular Carcinoma [CHALV1] antibody	250ug
Y050960	Anti-Human Hepatocellular Carcinoma [CHALV3] antibody	250ug
MO-T40006A	mAb anti-Human Hepatocellular Carcinoma Primary antibodies	0.5 mg
CS03-01-003	Human Hepatocellular carcinoma tissue array 5 (grade I ~ III)	7 x 9
Y050959	Anti-Human Hepatocellular Carcinoma [CHALV2] Antibody	100μg

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur

GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123

GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel: 0208-080893 Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62 SWIFT RABONL2U

GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur

ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.

ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX 058 710 33 48

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur

EN | FR | US |DE | PL
NL| BE | UK | BG