Did you know ? If you order before Friday 14h we deliver 90PCT of the the time next Tuesday, GENTAUR another in time delivery

Pubmed ID :24291177
Publication Date : //

Comparative evaluation of eleven commercial DNA extraction kits for real-time PCR detection of Bacillus anthracis spores in spiked dairy samples.


Spores of Bacillus anthracis are highly resistant and can survive conditions used for food preservation. Sample size and complexity represent the major hurdles for pathogen detection in food-related settings. Eleven commercial DNA extraction kits were evaluated for detection of B. anthracis spores by quantitative real-time PCR (qPCR) in dairy products. DNA was extracted from serial dilutions of B. anthracis spores in milk powder, cream cheese, whole milk and buttermilk. Three kits (QIAamp DNA mini kit, Invisorb Food kit I and II) were determined to produce the lowest limit of detections (LODs) with equally good performance. These kits employed lysozyme and proteinase K treatments or proteinase K in combination with cethyltrimethylamonium bromide-mediated (CTAB) precipitation of cell debris for cell disruption and DNA release. The LODs for these three kits were determined as 10(2) spores/ml of distilled water, 10(3)s pores/20 mg of powdered milk and 10(4) spores/100 mg of cream cheese, respectively. Performance testing of the QIAamp DNA mini kit demonstrated a good reproducibility and appropriate detection limits from 10(3)/ml for butter milk, 10(4)/ml for whole milk and 10(4)/100 mg for low fat cream cheese. However, DNA extraction efficiency was strongly inhibited by cream cheese with higher fat contents with an increased LOD of 10(6)/100 mg spores. This study demonstrated that qPCR detection depends directly on the appropriate DNA extraction method for an individual food matrix and bacterial agent.

Authors : Mertens Katja , Freund Lisa , Schmoock Gernot , Hänsel Christoph , Melzer Falk , Elschner Mandy C ,

Related products :

Catalog number Product name Quantity
TB101-50FRT RG,B Bacillus anthracis Real-TM Real Time Test with DNA extraction kit 50 tests
TB101-50FRT Bacillus anthracis Real-TM Real Time Test with DNA extraction kit 50 Tests
TVET23-50FRT Bacillus anthracis Real-TM Comulete Real Time Test with DNA extraction kit 50 Tests
TVET23-50FRT RG,iQ,SC,MX,A,B Bacillus anthracis Real-TM Complete Real Time Test with DNA extraction kit 50 tests
TB101-50FRT RG,B Bacillus anthracis Real_TM Real Time Test with DNA extraction kit 50 Tests 1 kit
FR056 Bacillus anthracis ,FLUORGENIC REALTIME PCR - READY TO USE KITS, REAL TIME PCR 100 REACTIONS
TVET23-50FRT Bacillus anthracis Real_TM Complete Real Time Test with DNA extraction kit 50 Tests RG,iQ,SC,MX,A,B_ Equipment tools see Cat. No TV_XXX 1 kit
B101-50FRT Bacillus anthracis Real-TM Real Time PCR kit 50 Tests
B101-50FRT RG,B Bacillus anthracis Real-TM Real Time PCR kit 50 tests
FR056 Bacillus anthracis REAL TIME PCR 100 REACTIONS
B101-50FRT RG,B Bacillus anthracis Real_TM Real Time PCR kit 50 Tests 1 kit
TV26-100FRT RG, iQ, MX, A, IL HPV High Risk Typing Real-TM Real Time Test with DNA extraction kit for qualitative detection and genotyping of 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 HPV 100 tests
TV26-100FRT Human Papilloma Viruses (HPV) High Risk Typing Real-TM Real Time Test with DNA extraction kit for qualitative detection and genotyping of 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 Human Papilloma 100 tests
TM 683 BRYANT AND BURKEY MEDIUM (for detection and enumeration of spores of lactate fermenting Clostridium in dairy products.) 500 gm
TB59-50FRT RG,iQ,SC,MX,A EHEC Real-TM Real Time Test with DNA extraction kit test for qualitative detection of enterohemorrhagic E.coli (EHEC) 50 tests
TV26-100FRT HPV High Risk Typing Real_TM CE Real Time Test with DNA extraction kit for qualitative detection and genotyping of 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 HPV 100 Tests RG, iQ, MX, A, IL_ Equi 1 kit
TV26-100FRT HPV High Risk Typing Real_TM CE Real Time Test with DNA extraction kit for qualitative detection and genotyping of 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 HPV 100 Tests RG, iQ, MX, A, IL_ Equi 1 kit
MKFR 014A Mycobacterium avium sp. paratuberculosis ,MINI KITS (REAL TIME PCR), REAL TIME PCR 25 REACTIONS
MKFR 007 Human hepatitis B virus ,MINI KITS (REAL TIME PCR), REAL TIME PCR 25 REACTIONS
MKFR 004 Bordetella pertusis ,MINI KITS (REAL TIME PCR), REAL TIME PCR 25 REACTIONS
MKFR 001 Leptospira pathogenic ,MINI KITS (REAL TIME PCR), REAL TIME PCR 25 REACTIONS
MKFR 003 Chlamydia pneumoniae ,MINI KITS (REAL TIME PCR), REAL TIME PCR 25 REACTIONS
TV1-100/2FRT RG, iQ, SC, MX, A HCV Real-TM Quant Real Time PCR Test with RNA_DNA extraction kit (25 ul Reaction Mix) 100 tests
TV7-100/2FRT C Cytomegalovirus (CMV) Real-TM Quant Real Time Test with with Column extraction kit 100 Tests
TV1-96FRT RG, iQ, MX, A HCV Real-TM Qual Real Time PCR Test with RNA_DNA extraction kit (50 ul Reaction Mix) 96 tests


 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: +49 0241 40 08 90 86, +49 0241 95 78 94 78, +49 0241 40 08 90 86
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur