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Index / Ray Biotech / Mouse Cystatin C ELISA ELISA / Product Detail : ELM-CystatinC-001 Mouse Cystatin C ELISA ELISA
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Jan
17th

#ELM-CystatinC-001 Mouse Cystatin C ELISA ELISA

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  Price : 606   EUR
688   USD
470   GBP
2548   Zloty
81160   JPY
4677   NOK
5012   SEK
686   CHF

Product name : Mouse Cystatin C ELISA ELISA

Catalog number : ELM-CystatinC-001

Quantity: One 96-Well Strip Microplate Kit

Availability: Yes

Supplier name : Ray Biotech

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About this Product :

Mouse Cystatin C ELISA ELISA
https://en.wikipedia.org/wiki/ELISA
note
RnD sytems have the most used ELISAs for cytokines but you could as wel use our Mouse Cystatin C ELISA ELISA if you don’t have to compare to former publications for your protein.

More Details about

Product Features

Sensitivity
10 pg/ml
Recommended Dilution (serum & plasma)
200-2,000 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Target Name/s

CystatinC

Species Detected

Mouse

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Specificity

The antibody pair provided in this kit recognizes mouse Cystatin C.

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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