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Index / Ray Biotech / Human_Mouse_Rat Phospho-Mek (S217_S221) and Total Mek ELISA ELISA / Product Detail : PEL-Mek-S217-002 Human_Mouse_Rat Phospho-Mek (S217_S221) and Total Mek ELISA ELISA
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Nov
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#PEL-Mek-S217-002 Human_Mouse_Rat Phospho-Mek (S217_S221) and Total Mek ELISA ELISA

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  Price : 572   EUR
649   USD
444   GBP
2404   Zloty
76579   JPY
4413   NOK
4729   SEK
647   CHF

Product name : Human_Mouse_Rat Phospho-Mek (S217_S221) and Total Mek ELISA ELISA

Catalog number : PEL-Mek-S217-002

Quantity: One 96-Well Strip Microplate Kit

Availability: Yes

Supplier name : Ray Biotech

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About this Product :

Human_Mouse_Rat Phospho-Mek (S217_S221) and Total Mek ELISA ELISA
opis kitu
## product_name ## po więcej informacji zapraszamy do kontaktu z naszym biurem w sopocie

Human_Mouse_Rat Phospho-Mek (S217_S221) and Total Mek ELISA ELISA mus musculus murine Human_Mouse_Rat Phospho-Mek (S217_S221) and Total Mek ELISA ELISA detects proteins from variouse species most likely human.
http://mybiofast.com/ver.php?search=musculus&submit=Search

More Details about

 

Product Feature

  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material
  • High sensitivity
  • High quality
  • Cost-effective
  • Complete kit with pre-coated 96 well strip-plate

Target Name

Mek-S217

Target Species

Human

Species Detected

Human, Mouse, Rat

Application Notes

Suggested Application
Quantitative Protein Detection; Establishing Normal Range
Kit Components
  • Pre-Coated 96-Well Strip Microplate
  • Wash Buffer
  • Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Other Materials Required
  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 μl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
  1. Prepare all reagents and samples as instructed in the manual.
  2. Add 100 μl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C.
  3. Add 100 μl of prepared primary antibody to each well. Incubate 1 h at RT.
  4. Add 100 μl of prepared 1X HRP-Streptavidin to each well. Incubate 1 h at RT.
  5. Add 100 μl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT.
  6. Add 50 μl of Stop Solution to each well. Read at 450 nm immediately.

Specificity

The antibody pair provided in this kit recognizes human, mouse and rat Phospho-Mek1 (Ser217/221) + pan Mek.

Storage/Stability

Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
 
 
 
 

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.

 

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