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Index / Biovis / Active Human Caspase_9100 units / Product Detail : 1089-100 Active Human Caspase_9100 units
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Feb
21th
#1089-100 Active Human Caspase_9100 units
Price : | 384 | EUR |
435 | USD | |
297 | GBP | |
1613 | Zloty | |
51387 | JPY | |
2961 | NOK | |
3173 | SEK | |
434 | CHF |
Product name : Active Human Caspase_9100 units
Catalog number : 1089-100
Quantity: 100 units
Availability: Yes
Supplier name : Biovis
Data sheet: Ask more or other datasheet now !
About this Product :
Active Human Caspase_9100 units Human samples 80 % of the research is conducted on human samples. GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Active Human Caspase_9100 units.http://mybiofast.com/ver.php?search=human+&submit=Search
More Details about
UNIT DEFINATION:
One unit of the recombinant caspase-9 is the enzyme activity that cleaves 1 nmol of the caspase substrate LEHD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.
FORM: Lyophilized powder
RECONSTITUTION: Reconstitute to 1 unit per l in PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 400 units/mg
PURITY: >90% by SDS-PAGE
STORAGE CONDITIONS:
The lyophilized caspase-9 is stable for 1 year at –70°C. Following reconstitution in PBS, the enzyme should be aliquoted and immediately stored at –70°C. Avoid multiple freeze/thaw cycles as activity might decrease.
DESCRIPTION:
Caspase-9 is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-9 also exists in cells as an inactive proenzyme. During the initiation of apoptosis procaspase-9 is processed at aspartate residues to form active caspase-9. As one of the initiator caspases, active caspase-9 functions to trigger activation of downstream effector caspases, leading to disassembly of cell structures.
The recombinant active human caspase-9 was expressed in E. coli. The active caspase-9 is routinely tested at BioVision for its ability to enzymatically cleave these two substrates Ac-LEHD-pNA (Cat. #1076-200) or Ac-LEHD-AFC (1075-200).
APPLICATIONS AND USAGE:
Active caspase-9 is useful in studying enzyme regulation, determining target substrates, screening caspase inhibitors, or as a positive control in caspase activity assays. We recommend using 1 unit/assay for analyzing caspase activity.
For a complete caspase-9 assay protocol, please refer to BioVision’s Caspase-9 Fluorometric or Colorimetric Assay Kits (Cat.#: K118 and K119).
For Research Use Only! Not to be used in humans.
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