GENTAUR Belgium BVBA BE0473327336 Voortstraat 49, 1910 Kampenhout BELGIUM Tel 0032 16 58 90 45
GENTAUR U.S.A Genprice Inc,Logistics 547 Yurok Circle, SanJose, CA 95123
Tel (408) 780-0908, Fax (408) 780-0908, sales@genprice.com

Index / Smart Serology / ErbB2 _ HER2 _ CD340 ELISA kit , Human ELISA kit / Product Detail : SEK10004 ErbB2 _ HER2 _ CD340 ELISA kit , Human ELISA kit
Related keywords:

Nov
15th

#SEK10004 ErbB2 _ HER2 _ CD340 ELISA kit , Human ELISA kit

Ask technical file .

  Price : 723   EUR
820   USD
561   GBP
3037   Zloty
96762   JPY
5577   NOK
5975   SEK
817   CHF

Product name : ErbB2 _ HER2 _ CD340 ELISA kit , Human ELISA kit

Catalog number : SEK10004

Quantity: 5 Plates

Availability: Yes

Supplier name : Smart Serology

ask pdf gentaur products Data sheet: Ask more or other datasheet now !

About this Product :

ErbB2 _ HER2 _ CD340 ELISA kit , Human ELISA kit
http://biotech-map.com/aa_search/table_sample.html?q=ELISA%20kits&filter=
Description
ErbB2 _ HER2 _ CD340 ELISA kit , Human ELISA kit is a high standard ELISA kit made with antibodies and plates of best quality as means for succesful acomplishment of your experiments and achievement of excellent and reliable results. For optimal conditions in every step of the protocol starting with the dilution of the samples, followed by incubation, blocking and washing, well designed buffers are included in the kit.

ErbB2 _ HER2 _ CD340 ELISA kit , Human ELISA kit Human samples 80 % of the research is conducted on human samples. GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as ErbB2 _ HER2 _ CD340 ELISA kit , Human ELISA kit.
http://mybiofast.com/ver.php?search=human+&submit=Search

More Details about

1. Materials provided

Capture Antibody - 0.5 mg/mL of mouse anti-ErbB2 monoclonal antibody. Dilute to a working concentration of  0.5 μg/mL in CBS before coating.

Detection Antibody - 0.28 mg/mL of biotinylated rabbit anti-ErbB2 polyclonal antibody. Dilute to a working concentration of 1.0 μg/mL in detection antibody dilution buffer before use.

Standard - Each vial contains 14ng of recombinant ErbB2. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -70℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of  1ng/mL  is recommended.

Streptavidin-HRP - 50 μL of streptavidin conjugated to horseradish-peroxidase. 1:2000  Dilution in detection antibody dilution buffer before use.

 

2. Sensitivity

The minimum detectable dose of human ErbB2 ( HER2 / CD340 ) was determined to be approximately 15.6 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

 

3. Principle of the product

The human ErbB2 ( HER2 / CD340 ) ELISA Pair Set is for the quantitative determination of human ErbB2.

This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. Each Set contains sufficient materials to run ELISAs on five 96-well plates.

The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for ErbB2 ( HER2 / CD340 ) coated on a 96-well plate. Standards and samples are added to the wells, and any ErbB2 present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-ErbB2 ( HER2 / CD340 ) polyclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”, which produces color in proportion to the amount of ErbB2 present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.

STORAGE - Keep streptavidin-HRP at 4℃ and protect it from prolonged exposure to light. Aliquot all other reagents and store at -20℃ to -70℃ in a manual defrost freezer.

ErbB2 / HER2 / CD340 ELISA PROTOCOL

 

1. Plate Preparation

  1. Dilute the capture antibody to the working concentration in CBS. Immediately coat a 96-well microplate with 100μL per well of the diluted capture antibody. Seal the plate and incubate overnight at 4℃.
  2. Aspirate each well and wash with at least 300μl wash buffer, repeating the process two times for a total of three washes. Complete removal of liquid at each step is 3. essential for good performance. After the last wash, remove any remaining wash buffer by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL of blocking buffer to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

 

2. Assay Procedure

  1. Add 100 μL of sample or standards in sample dilution buffer per well. Seal the plate and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of plate preparation.
  3. Add 100 μL of the detection antibody, diluted in antibody dilution buffer, to each well. Seal the plate and incubate 1 hour at room temperature.
  4. Repeat the aspiration/wash as in step 2 of plate preparation.
  5. Add 100 μL of Streptavidin-HRP to each well. Incubate for 1 hour at room temperature.
  6. Repeat the aspiration/wash as in step 2 of plate preparation.
  7. Add 200 μL of substrate solution to each well. Incubate for 20 minutes at room temperature ( if substrate solution is not as requested, the incubation time should be optimized ). Avoid placing the plate in direct light.
  8. Add 50 μL of stop solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm.

 

3. Calculation of results

  1. Calculate the mean absorbance for each set of duplicate standards, controls and samples. Subtract the mean zero standard absorbance from each.
  2. Construct a standard curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis and draw a best fit curve through the points on the graph.
  3. To determine the concentration of the unknowns, find the unknowns’ mean absorbance value on the y-axis and draw a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the x-axis and read the concentration. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
  4. Alternatively, computer-based curve-fitting statistical software may also be employed to calculate the concentration of the sample.

Contact us about this product :

Our team will respond you as soon as possible !

Email :
Skype :
Name :
Phone :
address :
Question, Comment ... :
arrow security gentaurPlease retype this code below:
Smart_Serology \ ErbB2___HER2___CD340_ELISA_kit_,_Human_ELISA_kit \ SEK10004
Reload Image

 

GENTAUR Belgium BVBA BE0473327336
Voortstraat 49, 1910 Kampenhout BELGIUM
Tel 0032 16 58 90 45

Fax 0032 16 50 90 45
info@gentaur.com | Gentaur | Gentaur





GENTAUR Ltd.
Unicorn House, Station Cl
Hertfordshire, Potters Bar EN6 1TL
Whetstone London N20 9BH
Tel 020 3393 8531 Fax 020 8445 9411
uk@gentaur.com | Gentaur | Gentaur

 

 




GENTAUR France SARL
9, rue Lagrange, 75005 Paris
Tel 01 43 25 01 50

Fax 01 43 25 01 60
RCS Paris B 484 237 888

SIRET 48423788800017
RIB 30004 00187 00010092253 10
BNP PARIBAS PARIS PL MAUBERT BIC BNPAFRPPPRG
IBAN FR76 3000 4001 8700 0100 9225 310
france@gentaur.com | Gentaur | Gentaur

GENTAUR GmbH
Marienbongard 20
52062 Aachen Deutschland
Support Karolina Elandt
Tel: 0035929830070
Fax: (+49) 241 56 00 47 88

Logistic :0241 40 08 90 86
Bankleitzahl 39050000
IBAN lautet DE8839050000107569353
Handelsregister Aachen HR B 16058
Umsatzsteuer-Identifikationsnummer *** DE 815175831
Steuernummer 201/5961/3925
de@gentaur.com | Gentaur | Gentaur

GENTAUR U.S.A
Genprice Inc, Logistics
547, Yurok Circle
San Jose, CA 95123
CA 95123
Tel (408) 780-0908,
Fax (408) 780-0908,
sales@genprice.com

Genprice Inc, Invoices and accounting
6017 Snell Ave, Ste 357
San Jose, CA 95123




GENTAUR Nederland BV
NL850396268B01 KVK nummer 52327027
Kuiper 1
5521 DG Eersel Nederland
Tel:  0208-080893  Fax: 0497-517897
nl@gentaur.com | Gentaur | Gentaur
IBAN: NL04 RABO 0156 9854 62   SWIFT RABONL2U






GENTAUR Spain
tel:0911876558
spain@gentaur.com | Gentaur | Gentaur






ГЕНТАУЪР БЪЛГАРИЯ
ID # 201 358 931 /BULSTAT
София 1000, ул. "Граф Игнатиев" 53 вх. В, ет. 2
Tel 0035924682280 Fax 0035924808322
e-mail: Sofia@gentaur.com | Gentaur | Gentaur
IBAN: BG11FINV91501014771636
BIC: FINVBGSF

GENTAUR Poland Sp. z o.o.


ul. Grunwaldzka 88/A m.2
81-771 Sopot, Poland
TEL Gdansk 058 710 33 44 FAX  058 710 33 48              

poland@gentaur.com | Gentaur | Gentaur

Other countries

Österreich +43720880899

Canada Montreal +15149077481

Ceská republika Praha +420246019719

Danmark +4569918806

Finland Helsset +358942419041

Magyarország Budapest +3619980547

Ireland Dublin+35316526556

Luxembourg+35220880274

Norge Oslo+4721031366

Sverige Stockholm+46852503438

Schweiz Züri+41435006251

US New York+17185132983

GENTAUR Italy
SRL IVA IT03841300167
Piazza Giacomo Matteotti, 6
24122 Bergamo Tel 02 36 00 65 93
Fax 02 36 00 65 94
italia@gentaur.com | Gentaur | Gentaur