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Index / Trevigen / PBR Protein (Recombinant) / Product Detail : 6360-025-01 PBR Protein (Recombinant)
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Nov
15th

#6360-025-01 PBR Protein (Recombinant)

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  Price : 153   EUR
173   USD
118   GBP
642   Zloty
20469   JPY
1179   NOK
1264   SEK
173   CHF

Product name : PBR Protein (Recombinant)

Catalog number : 6360-025-01

Quantity: 25 µl

Availability: Yes

Supplier name : Trevigen

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About this Product :

PBR Protein (Recombinant) recombinant proteins Most stable storage is at - 81 C or lower but some lyophilised proteins can be stored at +4C. Please read carefully the data sheet of the PBR Protein (Recombinant).
http://mybiofast.com/ver.php?search=rec.&submit=Search

More Details about

Recombinant Peripheral benzodiazepine receptor (PBR) protein


Catalog#: 6360-025-01

Size: 25 μl


Description: Recombinant full length mouse peripheral benzodiazepine receptor (PBR) purified from E. coli.


Applications: Control for Western Blot analysis using the anti-PBR antibody.


Physical State: This purified protein is provided at 0.2 mg/ml in 20 mM Tris 7.8, 1 mM EDTA, 0.1% Sodium Lauryl Sarcosine.


Expected Size: The recombinant protein is a fusion protein with a molecular weight of 20.4 kDa. Please note that multimers of the proteins are commonly seen on gels and Western Blotting Detection. The native protein is a 18 kDa protein.


Storage: Store at -20°C in working aliquots in a manual defrost freezer to avoid repeated freeze-thaws.
 

To use as a Western blot control:
Use 1 μg of the recombinant PBR protein per lane (5μl). Dilute the protein with 1X loading buffer of choice, supplemented with 2 mM DTT. Heat denature the control protein for 5 minutes before loading.



Fig.1 Western Blot analysis of PBR where 5 μl of the recombinant PBR protein was loaded on a 12% SDSPAGE gel, transferred to a PVDF membrane and detected with a 1:1000 dilution of the anti-PBR antibody (cat# 6362-PC-100). Detection was performed with a peroxidase-conjugated secondary antibody to rabbit followed by chemiluminescence.

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